The Power of Garlic
This is a companion blog post to my video on garlic, which you can view here.
In descending order of priority, the garlic supplements that I take are:
Life Extension Optimized Garlic (for allium content).
Swanson's Odor Controlled Garlic (for SAC content).
Kyolic Aged Garlic Extract Extra Strength Reserve (for other phytochemical content).
A. In terms of phenolic content mg/Kg, onion is superior to garlic, which is slightly superior to leeks. In terms of antioxidant status as judged by the DPPH assay, onions are superior to leeks, which are superior to garlic.
B. Garlic extract releases nitric oxide from S-nitrosoglutathione (GSNO) more than onion extract which does it more than leek extract.
GARLIC (Allium sativum)
A. Garlic contains over 100 secondary bio-active metabolites broadly categorized as organosulfur compounds, carotenoids, and polyphenols.
B. Organosulfur compounds:
a. When the garlic bulb is crushed, ground, or cut, the enzyme alliinase, together with the reactive intermediaries sulfenic acids, transform the amino acid alliin into allicin. Allicin is absent in the intact garlic bulb.
i. Allicin appears to be a defensive compound for the garlic plant, as it produces pain in predators by activating the TRPA1 and TRPV1 excitatory ion channels.
b. Allicin is a sulfur-containing, volatile compound. It is the most abundant thiosulfinate in fresh garlic, accounting for 70% of total thiosulfinates and 0.4% of the mass of fresh garlic. A single clove of garlic contains about 4-5 mg of allicin.
c. Allicin is highly lipophilic, allowing it to quickly pass through cell membranes, where it reacts with free thiol groups.
d. Allicin can be transformed into lipophilic organosulfur compounds, including diallyl disulfide (DADS – 67%, at room temperature), diallyl trisulfide (DATS – 15%), diallyl sulfide (DAS – 13%), and diallyl tertasulfide (5%).
e. Allicin can be transformed into hydrophilic organosulfur compounds, including S-allyl cysteine (SAC) and S-allyl mercaptocysteine (SAMC).
i. Allicin and SAC inhibit NFkB.
f. Allyl cysteine, alliin, allicin, and allyl disulfide scavenge free radicals differentially.
g. Allicin’s shelf-life can be increased with the addition of vinegar. At a lower pH of 5-6, allicin has a half-life of 10-17 days.
a. While onion has large amounts of quercetin (1497 mg/Kg), kaempferol (832 mg/Kg), and luteolin (391 mg/Kg), garlic has myricetin (693 mg/Kg), apigenin (217 mg/Kg), and quercetin (49 mg/Kg).
D. Fatty acids:
a. 23% of the lipids in garlic are phospholipids, while 14% are glycolipids and 63% are neutral lipids.
a. Flavonoid and phenolic content varies widely between cultivars.
b. Garlic varieties that contain florets or that are grown at higher altitudes tend to have higher S-allyl-L-cysteine sulfoxide (AlCSO), and those that are grown at lower altitudes tend to have higher phenolic contents.
A. Aged garlic is manufactured by soaking garlic in 12-20% aqueous ethanol for up to 20 months at room temperature, producing an extract that is filtered and concentrated at low pressure and temperature. This process converts the pungent odor of the water and lipid-soluble organosulphur compounds. It causes a loss in allicin though it increases the activity of S-allyl cysteine (SAC), S-allyl mercaptocysteine (SAMC), allixin, selenium, and N-fructosyl arginine.
B. Compared to fresh garlic extract:
a. Note on AGEs: Chronic hyperglycaemia (due to diabetes) produces protein glycation and eventually advanced glycation endproducts (AGEs), which cause tissue damage and oxidative stress and are responsible for many of the complications of diabetes. AGEs agonize the receptor for AGEs (RAGE).
b. Aged garlic extract is about 2x more capable at inhibiting cross-linked AGEs.
c. Aged garlic is more capable at inhibiting post-Amadori AGE formation at higher doses only.
d. About a 1/3rd better at inhibiting fructosamine formation.
e. Slightly worse at inhibiting glycation-induced protein carbonyl formation but better at inhibiting glycation-induced protein thiol formation.
f. Has a 1/3rd more antioxidant power on the ABTS (TEAC) assay (19 compared micrometer to 19.5 micrometer for vitamin C) and less (about 45% less) antioxidant power on the DPPH assay.
g. Fresh garlic is better at chelating iron ions than aged garlic.
h. In terms of phenolics, aged garlic has 129 mg/g whereas fresh garlic has 56 mg/g.
i. In terms of flavonoids, aged garlic has 101 mg/g whereas fresh garlic has 47 mg/g.
j. In terms of flavonols, aged garlic has 94 mg/g whereas fresh garlic has 43 mg/g.
GARLIC & INFLAMMATION
A. Garlic extracts improve the activity of glutathione.
B. Garlic powder extract inhibits NF-kB response to lipopolysaccharides in human blood.
C. A meta-analysis of 16 trials found that garlic reduced C-reactive protein by 0.61 mg/L in 13 trials, interleukin-6 by 0.73 ng/L in 5 trials, and tumor necrosis factor alpha by 0.26 ng/L in 7 trials.
a. Another meta-analysis found a reduction of 0.8 mg/L in C-Reactive Protein across 9 trials.
b. One meal of raw crushed garlic consumed alters the expression of genes relevant to immunity, apoptosis, and xenobiotic metabolism in human blood.
D. Allicin’s anti-inflammatory potential is so great that it prevents the destruction of cartilage in rodent models of osteoarthritis.
GARLIC & METABOLISM
A. Allicin increases the expression of uncoupling protein 1 (UCP1), likely indicating that it can increase the presence of brown adipose tissue (BAT).
B. Allicin appears to improve insulin resistance, decrease adiposity, and improve glucose homeostasis in obese rodents via BAT activation.
C. Allicin attenuates weight gain in a fructose-induced rodent model of the metabolic syndrome.
GARLIC, THE LIVER, AND KIDNEYS
A. Allicin improves glutathione peroxidase, glutathione reductase, and glutathione S-transferase activity in the liver.
B. Garlic extract appears to protect the liver from acetaminophen-induced damage.
C. In rodent models, allicin protects against NAFLD via its anti-inflammatory and antioxidant effects.
D. Allicin appears to protect kidneys from toxins in a similar manner.
GARLIC & DIABETES
A. Allicin appears to inhibit the activity of anti-islet antibodies present in type 1 diabetes.
GARLIC & CANCER
A. Epidemiological data indicates that allium vegetable intake, particularly intake of garlic and, to a lesser degree, onions, is inversely associated with gastric cancer incidence/mortality.
B. Epidemiological data also indicates that allium vegetable intake is inversely associated with prostate cancer incidence.
C. Allicin induces apoptosis in hepatocellular carcinoma cells.
D. Allicin induces apoptosis in glioma and glioblastoma cells.
E. Allicin inhibits telomerase and induces apoptosis in gastric cancer cells.
F. Allicin induces apoptosis in colon cancer cells (via Nrf2).
GARLIC & CARDIOVASCULAR HEALTH
A. It is thought that garlic’s cardio-protection is mediated by allicin and other organosulfur metabolites interacting with thiol groups and thiol-containing compounds (e.g. glutathione) to produce free hydrogen sulfide, which interacts with nitric oxide.
B. Garlic inhibits platelet aggregation by increasing cyclic nucleotides and by inhibiting fibrinogen binding and thus inhibiting platelet shape change.
C. A meta-analysis of 20 trials showed that garlic lowered systolic blood pressure by 5.1 mm Hg and lowered diastolic blood pressure by 2.5 mm Hg. This reduction appears to be evident only in hypertensive people.
D. A meta-analysis of 39 trials found garlic to reduce total cholesterol by 17 mg/dL and LDL-C by 9 mg/dL in people with total cholesterol of over 200 mg/dL, after a minimum of two months of use. This finding is less consistent than that on blood pressure.
E. Allicin prevents oxidized LDL from producing endothelial cell injuries by inhibiting apoptosis and oxidative stress.
F. Allicin inhibits cholesterol synthesis in the liver, while other metabolites also act on the synthesis differentially.
G. Allicin may be protective against atherosclerosis because of its inhibitory effect on TMAO synthesis.
GARLIC & THE BRAIN
A. Allicin may be neuroprotective from glutamate excitotoxicity. It has been shown to potently inhibit glutamate release in rodents’ brains.
B. Allicin is neuroprotective from traumatic brain injury. It exerts this neuroprotective effect by inhibiting oxidative damage and neuronal inflammation via increasing the phosphorylation of Akt and nitric oxide synthase.
C. Allicin is neuroprotective from ischemia-reperfusion (the return of blood supply following a hypoxic state) injury by inhibiting oxidative stress, inflammation, dysfunction of the mitochondrial respiratory chain, and apoptosis while upregulating antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase, and glutathione S-transferase.
D. Allicin dose-dependently inhibits both acetylcholinesterase and butyrylcholinesterase, thereby increasing the activity of acetylcholine in the brain.
E. In a rodent model of Alzheimer’s disease, allicin improves cognition by reducing oxidative stress, mitochondrial dysfunction, and reducing beta amyloid expression.
F. In a rodent model of cognitive impairment, allicin improves cognition by upregulating the Nrf2 antioxidant pathway.
G. In a rodent model of social defeat stress-induced depression, allicin attenuated depressive symptoms by reducing microglia activation, reducing inflammatory cytokine expression, reducing abnormal iron accumulation, and limiting neuronal apoptosis in the hippocampus.
GARLIC & HEAVY METAL POISONING
A. Allicin can potently and dose-dependently reduce the retention of lead (e.g. by up to 74% in the liver) in tissues and blood, though it also reduces zinc in tissues.
B. Garlic extract can also reduce retention of cadmium.
C. Allicin protects the liver from iron-induced damage.
D. Garlic extract protects against the cytotoxicity due to mercury poisoning.
E. Allicin protects the liver from damage due to arsenic poisoning.
GARLIC & THE MICROBIOME
A. Garlic exhibits antibacterial effects on gram-positive and gram-negative bacteria, as well as an ability to sensitive bacteria to antibiotics. It also exhibits anti-parasitic and anti-fungal effects. The chemical mechanism by which it exerts its antimicrobial effect remains unclear.
a. Inorganic polysulfides derived of organosulfur compounds are even more potent.
B. Kyolic aged garlic extract was found to increase the species of Lactobacillus and Clostridia after 3 months of supplementation.
C. Antimicrobial potential of garlic varies across genotypes and growing conditions.
D. Allicin reduces the transformation of L-carnitine into TMAO.
E. Allicin has proven to be useful in the treatment of Helicobacter pylori infections.
ALLICIN IN GARLIC & SUPPLEMENTS
A. Black garlic has less allicin than pickled garlic, which has less allicin than boiled garlic, which has less than roasted garlic. Enteric tablets had less bio-available allicin than non-enteric tablets, and enteric tablets were absorbed worse when consumed with a high-protein meal.
B. A study found that most garlic supplements produced less than 15% of the allicin they claimed to contain, mostly due to having low alliinase activity and not disintegrating quickly enough.
A. Note that one gram of fresh garlic should produce 1000 to 3333 mcg of allicin (1 to 3.33 mg).
a. Dried garlic should yield about 3x more allicin, since it is devoid of water.
B. Avoid: Garlique and Spring Valley (Walmart).
C. Lowest cost per mcg of allicin and potential allicin is Life Extension Optimized Garlic, which is 4.5x cheaper than the second cheapest (Nature’s Way Garlinase 5000).
D. Lowest cost per mcg of SAC is Swanson's Garlic Odor-Controlled, which is 4x cheaper than the second cheapest (Solgar Garlic Powder).
a. The cheapest aged garlic, per mcg of SAC, is Kyolic Aged Garlic Extract, which is 9x more expensive than Swanson’s.
E. ConsumerLab overall pick was Swanson's Garlic Odor-Controlled Garlic.
a. $0.07 per capsule.
b. 1 capsule provides 3060 mcg of alliin (0.61% of the powder) and 633 mcg of allicin (0.13% of the powder).
c. In total, 3 capsules a day procide 6030 mcg of allicin and 12,900 mcg of SAC.
F. However, Life Extension Optimized Garlic has far more allicin but far less SAC.
a. $0.095 per capsule.
b. Two capsules contain 57,400 mcg of alliin (4.8%) and 15,080 mcg of allicin (3.4%).
c. In total, two capsules provide 40,910 mcg of allicin and 224 mcg of SAC.
G. The top pick for aged garlic was Kyolic Aged Garlic Extract Extra Strength Reserve.
a. $0.2 per capsule.
b. In total, 2 capsules a day provide 3000 mcg of SAC (0.25%).
 Kavalcová, P., Bystrická, J., Tomáš, J., Karovičová, J., & Kuchtová, V. (2014). Evaluation and comparison of the content of total polyphenols and antioxidant activity in onion, garlic and leek. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 272-276.  Grman, M., Misak, A., Cacanyiova, S., Kristek, F., Tomaskova, Z., Bertova, A., & Ondrias, K. (2011). The aqueous garlic, onion and leek extracts release nitric oxide from S-nitrosoglutathione and prolong relaxation of aortic rings. General Physiology and Biophysics, 30(4), 396.  Lanzotti, V. (2006). The analysis of onion and garlic. Journal of chromatography A, 1112(1-2), 3-22.  Okada, Y., Tanaka, K., Fujita, I., Sato, E., & Okajima, H. (2005). Antiodidant activity of thiosulfinates derived from garlic. Redox Report, 10(2), 96-102.  Rybak, M. E., Calvey, E. M., & Harnly, J. M. (2004). Quantitative determination of allicin in garlic: supercritical fluid extraction and standard addition of alliin. Journal of Agricultural and Food chemistry, 52(4), 682-687.  Horev-Azaria, L., Eliav, S., Izigov, N., Pri-Chen, S., Mirelman, D., Miron, T., ... & Savion, N. (2009). Allicin up-regulates cellular glutathione level in vascular endothelial cells. European journal of nutrition, 48(2), 67-74.  Fujisawa, H., Suma, K., Origuchi, K., Seki, T., & Ariga, T. (2008). Thermostability of allicin determined by chemical and biological assays. Bioscience, biotechnology, and biochemistry, 72(11), 2877-2883.  Brodnitz, M. H., Pascale, J. V., & Van Derslice, L. (1971). Flavor components of garlic extract. Journal of Agricultural and Food Chemistry, 19(2), 273-275.  Rabinkov, A., Miron, T., Mirelman, D., Wilchek, M., Glozman, S., Yavin, E., & Weiner, L. (2000). S-Allylmercaptoglutathione: the reaction product of allicin with glutathione possesses SH-modifying and antioxidant properties. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1499(1-2), 144-153.  Bruck, R., Aeed, H., Brazovsky, E., Noor, T., & Hershkoviz, R. (2005). Allicin, the active component of garlic, prevents immune‐mediated, concanavalin A‐induced hepatic injury in mice. Liver International, 25(3), 613-621.  Ide, N., & Lau, B. H. (2001). Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-κB activation. The Journal of nutrition, 131(3), 1020S-1026S.  Chung, L. Y. (2006). The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide. Journal of medicinal food, 9(2), 205-213.  Wang, H., Li, X., Liu, X., Shen, D., Qiu, Y., Zhang, X., & Song, J. (2015). Influence of pH, concentration and light on stability of allicin in garlic (Allium sativum L.) aqueous extract as measured by UPLC. Journal of the Science of Food and Agriculture, 95(9), 1838-1844.  Lanzotti, V. (2006). The analysis of onion and garlic. Journal of chromatography A, 1112(1-2), 3-22.  Kamanna, V. S., & Chandrasekhara, N. (1980). Fatty acid composition of garlic (Allium sativum Linnaeus) lipids. Journal of the American Oil Chemists' Society, 57(6), 175.  Chen, S., Shen, X., Cheng, S., Li, P., Du, J., Chang, Y., & Meng, H. (2013). Evaluation of garlic cultivars for polyphenolic content and antioxidant properties. PLoS One, 8(11), e79730.  Hirata, S., Abdelrahman, M., Yamauchi, N., & Shigyo, M. (2016). Characteristics of chemical components in genetic resources of garlic Allium sativum collected from all over the world. Genetic resources and crop evolution, 63(1), 35-45.  Weiss, N., Ide, N., Abahji, T., Nill, L., Keller, C., & Hoffmann, U. (2006). Aged garlic extract improves homocysteine-induced endothelial dysfunction in macro-and microcirculation. The Journal of nutrition, 136(3), 750S-754S.  Dillon, S. A., Burmi, R. S., Lowe, G. M., Billington, D., & Rahman, K. (2003). Antioxidant properties of aged garlic extract: an in vitro study incorporating human low density lipoprotein. Life Sciences, 72(14), 1583-1594.  Borek, C. (2001). Antioxidant health effects of aged garlic extract. The Journal of nutrition, 131(3), 1010S-1015S.  Ryu, K., Ide, N., Matsuura, H., & Itakura, Y. (2001). N α-(1-deoxy-d-fructos-1-yl)-l-arginine, an antioxidant compound identified in aged garlic extract. The Journal of nutrition, 131(3), 972S-976S.  Elosta, A., Slevin, M., Rahman, K., & Ahmed, N. (2017). Aged garlic has more potent antiglycation and antioxidant properties compared to fresh garlic extract in vitro. Scientific reports, 7, 39613.  Perchellet, J. P., Perchellet, E. M., Abney, N. L., Zirnstein, J. A., & Belman, S. (1986). Effects of garlic and onion oils on glutathione peroxidase activity, the ratio of reduced/oxidized glutathione and ornithine decarboxylase induction in isolated mouse epidermal cells treated with tumor promoters. Cancer biochemistry biophysics, 8(4), 299-312.  Keiss, H. P., Dirsch, V. M., Hartung, T., Haffner, T., Trueman, L., Auger, J., ... & Vollmar, A. M. (2003). Garlic (Allium sativum L.) modulates cytokine expression in lipopolysaccharide-activated human blood thereby inhibiting NF-κB activity. The Journal of nutrition, 133(7), 2171-2175.  Darooghegi Mofrad, M., Milajerdi, A., Koohdani, F., Surkan, P. J., & Azadbakht, L. (2019). Garlic supplementation reduces circulating C-reactive protein, tumor necrosis factor, and Interleukin-6 in adults: a systematic review and meta-analysis of randomized controlled trials. The Journal of nutrition, 149(4), 605-618.  Taghizadeh, M., Hamedifard, Z., & Jafarnejad, S. (2019). Effect of garlic supplementation on serum C‐reactive protein level: A systematic review and meta‐analysis of randomized controlled trials. Phytotherapy research, 33(2), 243-252.  Charron, C. S., Dawson, H. D., Albaugh, G. P., Solverson, P. M., Vinyard, B. T., Solano-Aguilar, G. I., ... & Novotny, J. A. (2015). A single meal containing raw, crushed garlic influences expression of immunity-and cancer-related genes in whole blood of humans. The Journal of Nutrition, 145(11), 2448-2455.  Qian, Y. Q., Feng, Z. H., Li, X. B., Hu, Z. C., Xuan, J. W., Wang, X. Y., ... & Chen, J. X. (2018). Downregulating PI3K/Akt/NF-κB signaling with allicin for ameliorating the progression of osteoarthritis: in vitro and vivo studies. Food & function, 9(9), 4865-4875.  Lee, C. G., Rhee, D. K., Kim, B. O., Um, S. H., & Pyo, S. (2019). Allicin induces beige-like adipocytes via KLF15 signal cascade. The Journal of nutritional biochemistry, 64, 13-24.  Zhang, C., He, X., Sheng, Y., Xu, J., Yang, C., Zheng, S., ... & Zhai, B. (2020). Allicin regulates energy homeostasis through brown adipose tissue. Iscience, 101113.  Elkayam, A., Mirelman, D., Peleg, E., Wilchek, M., Miron, T., Rabinkov, A., ... & Rosenthal, T. (2003). The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. American journal of hypertension, 16(12), 1053-1056.  Wu, C. C., Chu, Y. L., & Sheen, L. Y. (2012). Allicin modulates the antioxidation and detoxification capabilities of primary rat hepatocytes. Journal of traditional and complementary medicine, 2(4), 323-330.  Anoush, M., Eghbal, M. A., Fathiazad, F., Hamzeiy, H., & SAEEDI, K. N. (2009). The protective effect of garlic extract against acetaminophen-induced loss of mitochondrial membrane potential in freshly isolated rat hepatocytes.  Panyod, S., Wu, W. K., Ho, C. T., Lu, K. H., Liu, C. T., Chu, Y. L., ... & Sheen, L. Y. (2016). Diet supplementation with allicin protects against alcoholic fatty liver disease in mice by improving anti-inflammation and antioxidative functions. Journal of agricultural and food chemistry, 64(38), 7104-7113.  El-Kashef, D. H., El-Kenawi, A. E., Suddek, G. M., & Salem, H. A. (2015). Protective effect of allicin against gentamicin-induced nephrotoxicity in rats. International immunopharmacology, 29(2), 679-686.  Abdel-Daim, M. M., Abushouk, A. I., Donia, T., Alarifi, S., Alkahtani, S., Aleya, L., & Bungau, S. G. (2019). The nephroprotective effects of allicin and ascorbic acid against cisplatin-induced toxicity in rats. Environmental Science and Pollution Research, 26(13), 13502-13509.  Osman, M., Adnan, A., Salmah Bakar, N., & Alashkham, F. (2012). Allicin has significant effect on autoimmune anti-islet cell antibodies in type 1 diabetic rats. Pol j pathol, 63(4), 248-254.  Guercio, V., Galeone, C., Turati, F., & La Vecchia, C. (2014). Gastric cancer and allium vegetable intake: a critical review of the experimental and epidemiologic evidence. Nutrition and cancer, 66(5), 757-773.  Hsing, A. W., Chokkalingam, A. P., Gao, Y. T., Madigan, M. P., Deng, J., Gridley, G., & Fraumeni Jr, J. F. (2002). Allium vegetables and risk of prostate cancer: a population-based study. Journal of the National Cancer Institute, 94(21), 1648-1651.  Chu, Y. L., Ho, C. T., Chung, J. G., Raghu, R., Lo, Y. C., & Sheen, L. Y. (2013). Allicin induces anti-human liver cancer cells through the p53 gene modulating apoptosis and autophagy. Journal of agricultural and food chemistry, 61(41), 9839-9848.  Li, C., Jing, H., Ma, G., & Liang, P. (2018). Allicin induces apoptosis through activation of both intrinsic and extrinsic pathways in glioma cells. Molecular medicine reports, 17(4), 5976-5981.  Cha, J. H., Choi, Y. J., Cha, S. H., Choi, C. H., & Cho, W. H. (2012). Allicin inhibits cell growth and induces apoptosis in U87MG human glioblastoma cells through an ERK-dependent pathway. Oncology reports, 28(1), 41-48.  Sun, L., & Wang, X. (2003). Effects of allicin on both telomerase activity and apoptosis in gastric cancer SGC-7901 cells. World Journal of Gastroenterology, 9(9), 1930.  Zhang, X., Zhu, Y., Duan, W., Feng, C., & He, X. (2015). Allicin induces apoptosis of the MGC-803 human gastric carcinoma cell line through the p38 mitogen-activated protein kinase/caspase-3 signaling pathway. Molecular medicine reports, 11(4), 2755-2760.  Bat-Chen, W., Golan, T., Peri, I., Ludmer, Z., & Schwartz, B. (2010). Allicin purified from fresh garlic cloves induces apoptosis in colon cancer cells via Nrf2. Nutrition and cancer, 62(7), 947-957.  Bradley, J. M., Organ, C. L., & Lefer, D. J. (2016). Garlic-derived organic polysulfides and myocardial protection. The Journal of nutrition, 146(2), 403S-409S.  Rahman, K., Lowe, G. M., & Smith, S. (2016). Aged garlic extract inhibits human platelet aggregation by altering intracellular signaling and platelet shape change. The Journal of Nutrition, 146(2), 410S-415S.  Ried, K. (2016). Garlic lowers blood pressure in hypertensive individuals, regulates serum cholesterol, and stimulates immunity: an updated meta-analysis and review. The Journal of nutrition, 146(2), 389S-396S.  Wang, H. P., Yang, J., Qin, L. Q., & Yang, X. J. (2015). Effect of garlic on blood pressure: A meta‐analysis. The Journal of Clinical Hypertension, 17(3), 223-231.  Ried, K., Toben, C., & Fakler, P. (2013). Effect of garlic on serum lipids: an updated meta-analysis. Nutrition reviews, 71(5), 282-299.  Chen, X., Pang, S., Lin, J., Xia, J., & Wang, Y. (2016). Allicin prevents oxidized low-density lipoprotein-induced endothelial cell injury by inhibiting apoptosis and oxidative stress pathway. BMC complementary and alternative medicine, 16(1), 1-6.  Gebhardt, R., Beck, H., & Wagner, K. G. (1994). Inhibition of cholesterol biosynthesis by allicin and ajoene in rat hepatocytes and HepG2 cells. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism, 1213(1), 57-62.  Gebhardt, R., & Beck, H. (1996). Differential inhibitory effects of garlic‐derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids, 31(12), 1269-1276.  Chen, C. P., Wu, W. K., Panyod, S., Wu, M. S., & Lee-Yan, S. (2019). Cardiovascular Disease Protective Effect of Allicin Through Gut Microbiota Modulation (FS07-08-19). Current developments in nutrition, 3(Supplement_1), nzz040-FS07.  Lu, C. W., Hung, C. F., Lin, T. Y., Hsieh, T. Y., & Wang, S. J. (2019). Allicin Inhibits Glutamate Release from Rat Cerebral Cortex Nerve Terminals Through Suppressing Ca2+ Influx and Protein Kinase C Activity. Journal of medicinal food, 22(7), 696-702.  Chen, W., Qi, J., Feng, F., Bao, G., Wang, T., Xiang, M., & Xie, W. F. (2014). Neuroprotective effect of allicin against traumatic brain injury via Akt/endothelial nitric oxide synthase pathway-mediated anti-inflammatory and anti-oxidative activities. Neurochemistry international, 68, 28-37.  Chen, W., Qi, J., Feng, F., Bao, G., Wang, T., Xiang, M., & Xie, W. F. (2014). Neuroprotective effect of allicin against traumatic brain injury via Akt/endothelial nitric oxide synthase pathway-mediated anti-inflammatory and anti-oxidative activities. Neurochemistry international, 68, 28-37.  Kumar, S. (2015). Dual inhibition of acetylcholinesterase and butyrylcholinesterase enzymes by allicin. Indian journal of pharmacology, 47(4), 444.  Zhang, H., Wang, P., Xue, Y., Liu, L., Li, Z., & Liu, Y. (2018). Allicin ameliorates cognitive impairment in APP/PS1 mice via suppressing oxidative stress by blocking JNK signaling pathways. Tissue and Cell, 50, 89-95.  Li, X. H., Li, C. Y., Lu, J. M., Tian, R. B., & Wei, J. (2012). Allicin ameliorates cognitive deficits ageing-induced learning and memory deficits through enhancing of Nrf2 antioxidant signaling pathways. Neuroscience letters, 514(1), 46-50.  Gao, W., Wang, W., Liu, G., Zhang, J., Yang, J., & Deng, Z. (2019). Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome. Metabolic brain disease, 34(1), 319-329.  Aslani, M. R., Najarnezhad, V., Mohri, M., & Azad, M. (2011). The effect of allicin on blood and tissue lead content in mice. Comparative Clinical Pathology, 20(2), 121-125.  Massadeh, A. M., Al-Safi, S. A., Momani, I. F., Alomary, A. A., Jaradat, Q. M., & AlKofahi, A. S. (2007). Garlic (Allium sativum L.) as a potential antidote for cadmium and lead intoxication: cadmium and lead distribution and analysis in different mice organs. Biological trace element research, 120(1-3), 227-234.  Liu, C. B., Wang, R., Dong, M. W., Wu, B., & Xiao, M. (2012). Protective effect of allicin against oxidative stress and hepatocyte autophagy in iron-overloaded rats. Zhongguo Yaolixue yu Dulixue Zazhi- Chinese Journal of Pharmacology and Toxicology, 26(4), 515-521.  Abdalla, F. H., Bellé, L. P., De Bona, K. S., Bitencourt, P. E. R., Pigatto, A. S., & Moretto, M. B. (2010). Allium sativum L. extract prevents methyl mercury-induced cytotoxicity in peripheral blood leukocytes (LS). Food and Chemical Toxicology, 48(1), 417-421.  Yang, D., Lv, Z., Zhang, H., Liu, B., Jiang, H., Tan, X., ... & Zhang, Z. (2017). Activation of the Nrf2 signaling pathway involving KLF9 plays a critical role in allicin resisting against arsenic trioxide-induced hepatotoxicity in rats. Biological trace element research, 176(1), 192-200.  Nakamoto, M., Kunimura, K., Suzuki, J. I., & Kodera, Y. (2020). Antimicrobial properties of hydrophobic compounds in garlic: Allicin, vinyldithiin, ajoene and diallyl polysulfides. Experimental and Therapeutic Medicine, 19(2), 1550-1553.  Xu, Z., Qiu, Z., Liu, Q., Huang, Y., Li, D., Shen, X., ... & Jiang, J. (2018). Converting organosulfur compounds to inorganic polysulfides against resistant bacterial infections. Nature communications, 9(1), 1-13.  Ried, K. (2020). Garlic lowers blood pressure in hypertensive subjects, improves arterial stiffness and gut microbiota: A review and meta-analysis. Experimental and Therapeutic Medicine, 19(2), 1472-1478.  Petropoulos, S., Fernandes, Â., Barros, L., Ciric, A., Sokovic, M., & Ferreira, I. C. (2018). Antimicrobial and antioxidant properties of various Greek garlic genotypes. Food chemistry, 245, 7-12.  Wu, W. K., Panyod, S., Ho, C. T., Kuo, C. H., Wu, M. S., & Sheen, L. Y. (2015). Dietary allicin reduces transformation of L-carnitine to TMAO through impact on gut microbiota. Journal of Functional Foods, 15, 408-417.  Si, X. B., Zhang, X. M., Wang, S., Lan, Y., Zhang, S., & Huo, L. Y. (2019). Allicin as add-on therapy for Helicobacter pylori infection: A systematic review and meta-analysis. World journal of gastroenterology, 25(39), 6025.  Lawson, L. D., & Hunsaker, S. M. (2018). Allicin bioavailability and bioequivalence from garlic supplements and garlic foods. Nutrients, 10(7), 812.  Lawson, L. D., & Wang, Z. J. (2001). Low allicin release from garlic supplements: a major problem due to the sensitivities of alliinase activity. Journal of agricultural and food chemistry, 49(5), 2592-2599.